【摘要】Acute myocardial infarction( AMI) is a major health problem leading to high rates of mortality and morbidity. Biomarker cardiac troponin I(cTnI) has shown high sensitivity and specificity towards AMI detection,and has been regarded as"gold standard". An ultrasensitive method to detectcTnI with low concentration in human fluid is essential. In this paper,we developed an aptamer-based assay coupled with rolling circle amplification( RCA) and molecular beacon probe for sensitive detection ofcTnI. In this strategy,aptamer acts as a bridge to communicate between oligonucleotides andcTnI. RCA reaction produces a single-stranded tandem repeated copy of the circular template,which are recognized by fluorescence molecular beacon probe. With this strategy,highly sensitive and specific detection of cTnI was realized with the lowest detectable concentration of 7. 24 pg/m L. The developed aptamer-RCA assay can be a promising tool in clinical samples analysis. The assay can also analyze other disease-related biomarkers by replacing the aptamer.
【关键词】
《建筑知识》 2015-05-12
《中国医疗管理科学》 2015-05-12
《中国医疗管理科学》 2015-05-12
《中国医疗管理科学》 2015-05-12
《铁道运营技术》 2015-06-25
《广西广播电视大学学报》 2015-07-01
《重庆高教研究》 2015-06-26
《重庆电子工程职业学院学报》 2015-07-02
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